Dynamic Light Scattering

Dynamic light scattering dls offers a complementary approach to sec for the characterization of protein complexes.

Dynamic light scattering. 1 dls technical note mrk656 01 introduction dynamic light scattering sometimes referred to as photon correlation spectroscopy or quasi elastic light scattering is a technique for measuring the size of particles typically in the sub micron region. It covers a broad size range from the lower nanometer range up to several micrometers. Dynamic light scattering dls sometimes referred to as quasi elastic light scattering qels is a non invasive well established technique for measuring the size and size distribution of molecules and particles typically in the submicron region and with the latest technology lower than 1nm. Dynamic light scattering dls is a measurement technique that provides a fast and simple method for submicron and nanoparticle sizing.

B dynamic light scattering. In the scope of dls temporal fluctuations are usually analyzed by means of the intensity or photon auto correlation function also known as photon correlation spectroscopy or quasi elastic light scattering. Sizes and size distributions in turn are calculated from the diffusion coefficients in terms of hydrodynamic radius r h or hydrodynamic diameter d h. Dynamic light scattering dls also known as photon correlation spectroscopy or quasi elastic light scattering is one of the most popular light scattering techniques because it allows particle sizing down to 1 nm diameter typical applications are emulsions micelles polymers proteins nanoparticles or colloids.

Light scattering is a phenomenon that is observed when light usually monochromatic laser light is scattered by randomly oriented objects in solution. The fluctuations are directly related to the rate of diffusion of the molecule through the solvent which is related in turn to the particles hydrodynamic radii. Dynamic light scattering dls is a technique in physics that can be used to determine the size distribution profile of small particles in suspension or polymers in solution. Dynamic light scattering is a well established standardized technique for particle size analysis in the nanometer range and has been used for about 40 years.

Dynamic light scattering dls is a robust simple and non contact method for the measurement of particle size and particle size distributions from the nanometre to the submicron range. Like sec it can also be used to determine the apparent molecular masses of protein complexes and is a useful tool for establishing complex stability. Theoretical basis for light scattering. With high sensitivity it is ideally suited for detection of size changes even as a function of time.

Dls provides information on the mean particle size as well as on particle size distribution. Size distributions without fractionation. In dynamic light scattering dls a k a. Quasi elastic light scattering qels the time dependent fluctuations in the scattered light are measured by a fast photon counter.

The sample is illuminated by a laser beam and the.